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A substrate enters the active site of the enzyme. Related to this is enzyme regulation where modification of an amino acid remote from the active site can control the activity of the enzyme.

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Enzymes do not start a chemical reaction but increase the rate of chemical reaction. It is the inhibition of enzyme activity by the presence of a chemical that competes with the substrate for binding to the active site of the enzyme.

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The activity of an enzyme primarily depends on its amino acid sequence of the protein chain. Substrate binding site along with the catalytic site form the active site of the enzyme.

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